Blue pippin.

For Nanopore sequencing, large fragments of genomic DNA were obtained using the Blue Pippin automatic nucleic acid fragment recovery system (Sage Science, MA), and barcodes were added using the Nanopore EXP-NBD104 kit.The final library was selected using the Blue Pippin protocol for fragments larger than 15 kb. Primer and polymerase were attached using the “Sequel Binding and Internal Ctrl Kit2.1” kit and purification was done using the PacBio “Procedure & Checklist—AMPure ® PB Bead Purication of Polymerase Bound SMRTbell ® …The BluePippin has the DNA capabilities of the Pippin Prep, plus the extra benefit of pulsed-field electrophoresis for resolving and collecting high molecular weight DNA. For long-range genomic applications, high-pass filtering allows users to collect all fragments above a size threshold set by the user.The Blue Wizards as seen by Ralph Damiani. The time that the Blue Wizards arrived in Middle-earth is uncertain. In Unfinished Tales, Tolkien wrote that the five Istari came to Middle-earth together in TA 1000. However, in The Peoples of Middle-earth, they are said to have arrived in the Second Age, around the year SA 1600, the time of the ...Blue Pippin and E-gel size selection systems; Agilent Bioanalyzer; Eppendorf epMotion M5073 liquid handling system; GTF Staff are available to answer questions regarding the use of technologies offered by the GTF and provide consultation on experimental design.

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the Pippin with the cover closed. Humidity from the cassette may cause damage to electrodes. Approximate Run Times for "Tight" Range Targets (hr:min) Run 1. Close the lid, go to the Main Tab, and make sure the proper protocol is loaded in the "Protocol Name" field. 2. Press "START". The run will automatically stop when

Blue Pippin and E-gel size selection systems; Agilent Bioanalyzer; Eppendorf epMotion M5073 liquid handling system; GTF Staff are available to answer questions regarding the use of technologies offered by the GTF and provide consultation on experimental design.98 subscribers Subscribe 16K views 11 years ago This video demonstrates how Pippin Prep and BluePippin from Sage Science (www.sagescience.com) perform automated DNA size selection for the...Jan 5, 2012 · 98 subscribers Subscribe 16K views 11 years ago This video demonstrates how Pippin Prep and BluePippin from Sage Science (www.sagescience.com) perform automated DNA size selection for the... Libraries are run through 10-15 cycles of PCR with Kapa Biosystems Library Amplification kits, followed by further size selection with Blue Pippin Prep (Sage Science). Final library quantification is achieved using the Qubit Fluorometer (for concentration) and the Agilent 2100 Bioanalyzer (for library insert size and length).Authentic Scottie Pippen Chicago Bulls jerseys are at the official online store of the National Basketball Association. We have the Official Bulls City Edition jerseys from Nike and Fanatics Authentic in all the sizes, colors, and styles you need. Get all the very best Chicago Bulls Scottie Pippen jerseys you will find online at store.nba.com.

Sheared genomic DNA was treated for damage repair and end repair, adaptor ligation, and size selection with a Blue Pippin system (Sage Science) to create SMRTbell template libraries.

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Size select the DNA with Blue Pippin, using High Pass Plus gel cassette and 0.75% Dye Free for 1-10 kb External Standards S1 according to manufacturer's protocol. Further, 10 μg of DNA is required for each lane, and typically the amplified sample is enough for two lanes. Adjust the cutoff length depending on the fragment size distribution.Internal price (Cornell and Cornell affiliates) External price. Fragment analysis, ready-to-load, per 96-well plate. $42.50. $69.70. LIZ500 and HiDi Formamide addition, per 96-well plate. $40.00. $65.60. ROX500 and HiDi Formamide addition, per 96-well plate. Sep 19, 2018 · The High Pass Plus cassette has a stocky separation column, so DNA has a shorter distance to travel. The wider column and increased taper provide higher resolution for a cleaner and more accurate size cut-off. The larger sample wells now allow a maximum load of 10ug (100% up from standard Blue Pippin cassettes). The entire elution was size selected on a 3% agarose cassette using the Blue Pippin system, targeting either 190 or 200 bp using "tight" settings. The 40 μL elution was collected from blue pippin and analyzed using High Sensitivity DNA bioanalyzer. Dilution is usually not necessary for this pool, but think about your expected concentration ...BEVERLY, Mass. — November 11, 2014 — Sage Science, a developer of life science products for improving sample preparation processes in molecular biology applications, today introduced the PippinHT, a high-throughput version of the popular Pippin line of automated DNA size selection instruments for use with next-generation sequencers.Pippin Prep DNA Size Selection System Selects 100 bp – 1.5 kb. SageELF Whole Sample Fractionation System DNA (up to 40 kb) or Proteins (up to 500 kDa). Pippin Pulse ...

The BluePippin produces higher quality DNA, improving downstream analysis. Pre-cast agarose gel cassettes are constructed in a way that eliminates cross contamination. WHY use the BluePippin? (Advantages) The CGRB houses multiple Illumina and Pacific Biosciences next generation sequencing platforms that all have applications that benefit from ...The blue-rumped parrot (Psittinus cyanurus) is a parrot found in the very southern tip of Myanmar, peninsular Thailand, Malaysia, Borneo, Sumatra and nearby ...First-strand cDNA was synthesized using a SMARTer PCR cDNA Synthesis Kit (Clontech, CA, United States). After a few rounds of PCR amplification, the amplified cDNA was divided into different size fractions to prevent preferential small template sequencing using a Blue Pippin (Sage Science, MA, United States).Empress plum. Empress, also widely-known as Blue Imperatrice, is an unusually late-season dark blue / purple European plum. The plums have a sweet sugary flavor, but the golden yellow flesh is quite dry. The plums are "persistent", and if not picked will hang on the tree well into late fall. They can also be kept in a fridge for several weeks.Sage Science pacbio sequencing with bluepippin Pacbio Sequencing With Bluepippin, supplied by Sage Science, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations.Blue Pippin Size-Selection System. The first step of the protocol was cDNA synthesis with adapters. and PCR cycle optimization followed by large-scale PCR amplification. PCR products were then size.最主要的是普通的切胶回收只能回收中小片段，不能回收三代平台所需的大片段。. 因此手工切胶回收正在被其他更安全简便的方法替代。. 其次是磁珠分选，磁珠分选在二代测序中应用比较广泛。. 原理是磁珠会优先抓取较大的核酸片段，在分选过程中，通过 ...

Libraries are run through 10-15 cycles of PCR with Kapa Biosystems Library Amplification kits, followed by further size selection with Blue Pippin Prep (Sage Science). Final library quantification is achieved using the Qubit Fluorometer (for concentration) and the Agilent 2100 Bioanalyzer (for library insert size and length).

1. Blue Pippin Start up 1.1. Powering up the Blue Pippin. The Blue Pippin instrument has to be rebooted each time it is used in order to connect to the server. Once it is turned off it loses the connection. Either reboot or if the device is turned off prior to use, locate the black power button at the rear of Blue Pippin. Push the button and ...pippin: [noun] a crisp tart apple having usually yellow or greenish-yellow skin strongly flushed with red and used especially for cooking.Repaired DNAs were selected by a Blue Pippin system to construct SMRTbell DNA libraries with fragment sizes of >10 kb according to the manufacturer's specification (PacBio, Menlo Park, CA, United States). Library quality was determined using a Qubit 2.0 Fluorometer (Life Technologies, CA, United States), and the average fragment size ...BluePippin Operations Manual 460013 Rev D 1-1 1 Introduction Thank you for purchasing the BluePippin from Sage Science. The BluePippin is anRun times are as little as 30 minutes. To resolve very large DNA fragments, pulsed-field runs may last several hours. View the Blue Pippin cassette reference chart to explore the DNA size selection options and run times. Kits include 10 ea. disposable pre-cast gel cassettes. All necessary standards and reagents are included with the purchase.The published protocol 19 was used with minor modifications: size selection was performed using a Blue Pippin (Sage Science). In some instances we used rnh201-RED instead of rnh201::kan (see results).The Blue Pippin facilitates library construction for most popular NGS platforms, and is recommended by Pacific Biosciences, Illumina and Ion Torrent for certain workflows. Utilizing pulsed-field electrophoresis it is an ideal solution for resolving and collecting high molecular weight DNA up to 50kb. The platform features the ability to collect ...The larger sample wells now allow a maximum load of 10 μg (100% up from standard Blue Pippin cassettes). Also the size of the elution module has been increased as well as the surface area of the filtration membrane, bringing about improved sample recovery and reproducibility. Blue Pippin software requirement: v6.31/6.40 CD31

The Blue Pippin is utilized for DNA size selection (up to 50kb) for NGS. Desired target sizes or ranges of sizes are entered in software and fractions are collected in buffer. Up to 5 samples/gel cassette may be run, with no possibility of cross contamination....

The library was size selected (15-80 kb) using the Blue Pippin size selection instrument (Sage Science) and then sequenced on six SMRT cells (one cell at 2 pM and five cells at 6 pM) using 1 M ...

I've found that if I use 3 serial 80% washes, my DNA is retained and I don't have salt problems in the Blue Pippin. ( N.B. I deliberately leave a little excess behind in the first 2 washes to ...Find many great new & used options and get the best deals for Sage Science BluePippin BR02552 50bp-30kb DNA Size Selection System Blue Pippin at the best online prices at eBay! Free shipping for many products!The PippinHT system is the top choice for higher-throughput workflows. The PippinHT can process 11 size selections at once on a gel cassette (1 of the 12 lanes must be dedicated to an external size marker). The PippinHT accommodates 2 gel cassettes so up to 22 samples can be run at once, and at a quarter of the run time (1 hr 15 m) when ...E. coli MG1655 P5C3 SAMN02743420 ammonium acetate or SDS, proteinase K, phenol-chloroform 17 none Blue Pippin (7 kb) S. cerevisiae 9464 P4C2 SAMN02731377 Qiagen genomic DNA buffer set 40 g-TUBE ...Cells were then counted using trypan blue staining. In total, 6,000 cells per well of a 96-well ultra-low attachment plate (Corning) were seeded. Once the plate was seeded, it was spun down at 234 ...In eukaryotes, alternative splicing (AS) is a crucial regulatory mechanism that modulates mRNA diversity and stability. The contribution of AS to stress is known in many species related to stress, but the posttranscriptional mechanism in poplar under cold stress is still unclear. Recent studies have utilized the advantages of single molecular real-time (SMRT) sequencing technology from Pacific ...We’ve taken the pulsed-field power supply out of the BluePippin instrument to provide researchers an affordable tool to resolve DNA out to 100 kb and beyond. The Pippin Pulse features a simple software interface to provide users full flexibility to develop their own field-inversion protocols. The operating range is 25-175V, and up to 200 mA ...With You Lyrics. [PIPPIN] My days are brighter than morning air. Evergreen pine and autumn blue. But all my days were twice as fair. If I could share my days with you. My nights are warmer than ...BN124141 Pippin Product Line Brochure (4 pages): Flyers: BN0205147. Paired-end sequencing flyer. for Illumina platforms. BN0205148. Pippin Pulse flyer. Pulsed-Field for gel boxes. BN0205149.Both approaches produced similar total yields of high-quality reads (roughly 3.5 gigabases, as shown by the point at which each line in Fig. 1C crosses the y-axis) but the yield of reads greater than 20KB was clearly higher when using the Blue Pippin, as shown by the red line in Figure 1C being higher than the blue line at a value of 20KB on ...

I've found that if I use 3 serial 80% washes, my DNA is retained and I don't have salt problems in the Blue Pippin. ( N.B. I deliberately leave a little excess behind in the first 2 washes to ...According to the manufacturer's protocols of the Blue Pippin system (Pacific Biosciences), fragments with sizes larger than 10 kb were selected. The quality and average size of the fragments of the library were estimated using a Qubit 2.0 fluorometer (Life Technologies, Carlsbad, CA, USA) and Bioanalyzer 2100 (Agilent Technologies, Santa ...VTG Chicago Bulls Scottie Pippen #33 Champion Jersey Mens 44 Red NBA 90s Y2K. $120.00. Free shipping. or Best Offer. SPONSORED. Vintage #33 CHICAGO BULLS Champion NBA Basketball Jersey. Sz. M 10-12.Instagram:https://instagram. hygear suspensionku jobs lawrencesamantha denise wimberley odessa txgianni bernini bag Blue Pippin. Sort by. Blue Pippin . More. Blue Pippin Cassette 3%, 100-250 bp, Internal Marker Q3 . CHF 818.00 . CHF 818.00 . Add to cart More. Blue Pippin Cassette 2%, 100-600 bp, Internal Marker . CHF 818.00 . CHF 818.00 . Add to cart More. Blue Pippin Cassette 2%, 100-600 bp, External Marker . CHF 818.00 . CHF 818.00 ...As researchers engage larger-scale genomic and transcriptomic studies, the need for high-quality NGS libraries is greater than ever. Now we can provide Pippin size selection for high-throughput workflows so you can easily scale up your sample prep with speedy run times. Features of the PippinHT: 2 x 12-sample gel cassettes can be run at one time. kansas university rosteramerican dream art If you'd like us to Pippin your samples for you (plus perform quantification and fragment analysis), the cost is $75 per sample. CLICK HERE to schedule a reservation on BluePippin! (Note, you will need to register for an iLab account.) Fragment Analyzer traces of 5 different Blue Pippin size selected Illumina libraries. wnit basketball If you’re in the market for a new or used boat, one valuable tool that can help you negotiate the best price is a blue book for boats. Before diving into negotiating a price using a blue book for boats, it’s important to have a good underst...Blue Pippin Productions first offering answers this question. When a pregnant wife confronts her husband about his neglect and infidelity, there are disastrous results. A busybody neighbor's chance interruption only delays the outcome of this Fish Story. Check it out on IMDB!A Blue Pippin device (Sage Science, Inc., Beverly, MA, USA) was used to size select the SMRT Bell template and enrich large fragments (>10 kb). The size-selected library was quality inspected and quantified on an Agilent Bioanalyzer 12 kb DNA Chip (Agilent Technologies, Santa Clara, CA, USA) and a Qubit fluorimeter (Invitrogen, Carlsbad, CA ...